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ASSESSMENT OF GENETIC (VIA PROTEIN ELECTROPHORESIS) AND NUTRITIONAL VARIABILITIES AMONG TEN ACCESSIONS OF SPHENOSTYLIS STENOCARPA (HOCHT. EX. A. RICH) HARMS

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TABLE OF CONTENTS
Title page – – – – – – – – i
Certification page – – – – – – – – ii
Dedication – – – – – – – – iii
Acknowledgement – – – – – – – – iv
Table of contents – – – – – – – – v
List of plates – – – – – – – – vii
List of figures – – – – – – – viii
List of tables – – – – – – – – ix
Abstract – – – – – – – – x
CHAPTER ONE: INTRODUCTION – – – – – 1
CHAPTER TWO: LITERATURE REVIEW – – – 8
CHAPTER THREE: MATERIALS AND METHODS – – 14
3.1. SODIUM DODECYL SULPHATE – POLYACRYLAMIDE GEL
ELECTROPHORESIS (SDS-PAGE) – – – – 14
3.1.1. Protein extraction – – – – – – 14
3.1.2 Preparation of gel – – – – – – 15
3.1.3 Loading the electrophoresis tank – – – – 16
3.1.4. Loading the samples – – – – – 16
3.1.5 Staining the gel – – – – – – 16
3.1.6 Destaining – – – – – – 17
3.1.7 Gel documentation and analysis – – – – 17
3.2. NUTRITIONAL ANALYSIS – – – – – 18
3.2.1. PROXIMATE ANALYSIS – – – – 18
vi
3.2.1.1.Determination of crude protein content – – – 18
3.2.1.2Determination of carbohydrate content – – – 19
3.2.1.3Determination of crude fat content – – – 19
3.2.1.4.Determination of crude fiber content – – – 19
3.2.1.5Determination of ash content – – – – 20
3.2.1.6 Determination of moisture content – – – 20
3.3.1 MINERALS AND VITAMIN ANALYSIS – – – 21
3.3.1.1.Determination of Vitamin A – – – – – 21
3.3.1.2.Determination of Vitamin B – – – – 21
3.3.1.3Determination of Vitamin C – – – 22
3.3.1.4Determination of Zinc – – – – 22
3.3.1.5Determination of Lead – – – – – 22
3.3.1.6Determination of Iron – – – – – 22
3.4. DATA ANALYSIS – – – – – – – 23
CHAPTER FOUR: RESULTS – – – – – – 31
4.1. Evaluation of Genetic variability – – – – – 31
4.2. Nutritional Variability – – – – – – 32
CHAPTER FIVE: DISCUSSION AND CONCLUSION – – 42
CONCLUSION – – – – – – – – 50
REFERENCES – – – – – – – – 51
APPENDICES –

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