Placeholder

CHARACTERIZATION OF PENTACLETHRA MACROPHYLLA BENTH. THROUGH PROTEIN ELECTROPHORESIS

Topic Description

TABLE OF CONTENT
Title page ————————————————————————————— i
Certification page ——————————————————————————— ii
Dedication —————————————————————————————- iii
Acknowledgment ——————————————————————————- iv
Table of Contents ——————————————————————————– v
List of Plates ————————————————————————————- vii
List of Figures ———————————————————————————- viii
List of Tables ———————————————————————————- ix
Abstract ——————————————————————————————- x
CHAPTER ONE: INTRODUCTION —————————————————— 1
CHAPTER TWO: LITERATURE REVIEW ——————————————– 10
CHAPTER THREE: MATERIALS AND METHODS ——————————- 17
3.1.1 Plant material ——————————————————————— 17
3.1.2 Protein extraction —————————————————————– 19
3.1.3 Protein estimation —————————————————————- 19
6
3.1.4 Reagents ————————————————————————— 19
3.2 SODIUM DODECYL SULPHATE POLYACRYLAMIDE GEL
ELECTROPHORESIS (SDS-PAGE) ———————————————— 19
3.2.1 Gel preparation ————————————————- —————— 19
3.2.2 Resolving gel composition —————————————————— 20
3.2.3 Stacking gel composition ——————————————————– 20
3.2.4 Loading the electrophoretic tank ———————————————- 20
3.2.5 Loading the samples and electrophoresis ————————————– 21
3.2.6 Staining the gel ——————————————————————– 21
3.2.7 Destaining ————————————————————————– 22
3.2.8 Gel documentation and analysis ———————————————– 22
CHAPTER FOUR: RESULTS
4.1 Evaluation of genetic variability ————————————————– 23
CHAPTER FIVE: DISCUSSION AND CONCLUSION —————————— 32
REFERENCES ——————————————————————————— 37

GET COMPLETE PROJECT